Thesis Archive
Saccharification of Microwave Pretreated Kenaf (Hibiscus Cannabinus) Using Rumen Fluid (2012)
Impas, Isobel Rae
Tan, Rachiel Ruth
Young, Mark Reinhard
Abstract:
-In this study, rumen fluid was used as the source of enzyme for saccharification of Kenaf bast fiber (KBF). Furthermore, this study introduced microwave pretreatment as its primary pretreatment method. Preliminary experiments were done to obtain the best values of the rumen fluid (RF) and substrate concentrations which were found to be 3% (v/v) and 10 g/L respectively among values of 1%, 3%, and 5% (v/v) for enzyme concentration and 10, 20, and 30 g/L for the substrate concentration. Process parameters for microwave pretreatment were identified to be the irradiation time and fiber concentration. 32 factorial design was used with parameter values of 10, 20, and 30 minutes irradiation time and 20, 30, 40 g/L fiber concentration. The highest sugar yield was found when irradiation time is 10 minutes with fiber concentration of 20 g/L with the value of 0.0318 g total sugar per gram kenaf bast fiber, 3.83% higher than the untreated fiber. Though the obtained total sugar yield is minimal, further research is encouraged to investigate possible solutions to increase total sugar yield.
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