Thesis Archive
MICROWAVE-ASSISTED EXTRACTION OF SINIGRIN FROM MUSTARD (Brassica juncea) SEED MEAL
Patricia Shaine B. Gonzales
Dorisse Ann F. Laygo
Zoe Aurora H. Romero
Abstract:
Glucosinolates, commonly found in Brassica plants, are compounds derived from glucose and amino acid. Classified under the Brassicacease family, mustard is among one of the species containing the highest concentration of glucosinolates. Recent studies have reported the beneficial effects of consuming glucosinolate-containing food due to the substance’s chemopreventive characteristics. Glucosinolate compounds can be isolated using a variety of conventional and non-conventional methods. Microwave-assisted extraction (MAE) is one of the non-conventional methods that offers advantages such as high purity of desired compounds and reduction of extraction time.
The study aims to isolate the abundant type of glucosinolate, sinigrin, from mustard seed meal (Brassica juncea) utilizing MAE. Specifically, the effects of three main parameters, solvent-to-solid ratio (20:1 mL/g, 30:1 mL/g, and 40:1 mL/g), extraction time (5 min, 7.5 min, and 10 min), and microwave power (140 W, 420 W, and 700 W), were investigated while the subsequent yield was optimized.
Upon analysis, the sinigrin yield from the study ranges from 18.66 to 40.23 mg sinigrin/g seed meal. The optimum values of the extraction parameters within the range of the study was determined to be 156.77 W, 10 min extraction time, and a solvent-to-solid ratio of 20:1 mL/g seed meal. The predicted yield was 35.98 mg sinigrin/g seed meal with a desirability of 0.8315 while the actual sinigrin yield was 32.07 mg sinigrin/g seed meal and the total glucosinolate yield was measured to be 86.06 μmol sinigrin equivalent/g seed meal.
Microwave-assisted extraction is suggested to be a good extraction method for glucosinolate in mustard seed meal as the yield of total glucosinolates from the study was observably higher than previous studies. However, additional studies on different range of parameters are recommended in order to further improve the extraction yield.
Adviser:
Dr. Cynthia F. Madrazo