Bridges: Dialogues Towards a Culture of Peace

BRIDGES: Dialouges Towards a Culture of Peace



bridges logo: dialogues towards a culture of peace

Prof. Ada E. Yonath


2009 Nobel Laureate for Chemistry

Public keynote speech and dialogue
De La Salle University
March 2, 2015

Topic: From basic science to advanced medicine – Molecules of life and their impact on modern biomedical research

 

Abstract:

Striving to understand the process of the translation of the genetic code, a basic and most important process of life, we determined the structure of the ribosome, the universal cellular “factory” that performed the formation of the proteins (the cell workers) according to the genetic code and deciphered its mode of function. Owing to their vital role in cell life, the ribosomes are targeted by many antibiotics, which paralyze them by binding to their functional sites. Consequently the antibiotics binding modes, inhibitory actions and synergism pathways have been determined for almost all ribosomal antibiotics. These indicated the principles of differentiation between patients and pathogens, suggested mechanisms leading to bacterial resistance and paved ways for improvement of existing antibiotics as well as for the design of advanced therapeutics capable of minimizing antibiotics resistance.

 

Biography:

Prof. Ada E. Yonath is a 2009 Nobel Laureate for Chemistry at the Weizmann Institute of Science in Israel who has shown what the ribosome – the cell’s “protein factory” - looks like, how it functions at the atomic level and how it translates DNA information into proteins, which perform most of life processes.

An understanding of the ribosome's innermost workings is important for a scientific understanding of life. This knowledge can be put to a practical and immediate use: many of today's antibiotics cure various diseases by blocking the function of bacterial ribosomes. Without functional ribosomes cells of all organisms including bacteria cannot survive. This is why ribosomes are such an important target for new antibiotics.

Professor Yonath has generated 3D models that show how different antibiotics bind to the ribosome and how bacteria become resistant to antibiotics. These models have revolutionized the field of structural biology and are now used by scientists in order to develop new antibiotics, directly assisting the saving of lives.

Graduating from the Hebrew University of Jerusalem with a bachelor's degree in chemistry in 1962 and a master's degree in biochemistry in 1964, Ada Yonath earned a Ph.D. in X-Raycrystallography at the Weizmann Institute of Science in 1968. She accepted postdoctoral positions at Carnegie Mellon University (1969) and MIT (1970). While a postdoc at MIT she spent some time in the lab of subsequent 1976 Chemistry Nobel Laureate William N. Lipscomb, Jr. of Harvard University where she was inspired to pursue very large structures.

In 1970 Professor Yonath established the first protein crystallography laboratory in Israel. She was visiting professor at the University of Chicago in 1977-78 and from 1979 to 1984 was a visiting scientist with Heinz-Günter Wittmann at the Max Planck Institute for Molecular Genetics in Berlin. She headed a Max-Planck Research Unit at DESY in Hamburg (1986–2004) in parallel to her research activities at the Weizmann Institute.

In her work Professor Yonath focuses on the mechanisms underlying protein biosynthesis by ribosomal crystallography, a research line she pioneered over twenty years ago despite considerable skepticism of the international scientific community. Ribosomes translate RNA into protein and because they have slightly different structures in microbes, when compared to eukaryotes, such as human cells, they are often a target for antibiotics.

Professor Yonath determined the complete high-resolution structures of both ribosomal subunits and discovered within the otherwise asymmetric ribosome the universal symmetrical region that provides the framework and navigates the process of polypeptide polymerization. Consequently she showed that the ribosome is a ribozyme that places its substrates in stereochemistry suitable for peptide bond formation and for substrate-mediated catalysis. Two decades ago she visualized the path taken by the nascent proteins, namely the ribosomal tunnel and recently revealed the dynamics elements enabling its involvement in elongation arrest, gating, intra-cellular regulation and nascent chain trafficking into their folding space.

Additionally, Professor Yonath elucidated the modes of action of over twenty different antibiotics targeting the ribosome, illuminated mechanisms of drug resistance and synergism, deciphered the structural basis for antibiotic selectivity and showed how it plays a key role in clinical usefulness and therapeutic effectiveness, thus paving the way for structure-based drug design. For enabling ribosomal crystallography Professor Yonath introduced a novel technique, cryo bio-crystallography, which became routine in structural biology and allowed intricate projects otherwise considered formidable.

At the Weizmann Institute Professor Yonath is the incumbent of the Martin S. and Helen Kimmel Professorial Chair. She is the 1st Israeli woman to win the Nobel Prize, the 1st woman from the Middle East to win a Nobel Prize in science and the 1st woman in 45 years to win the Nobel Prize for Chemistry.