iCELL

Laboratory

The DLSU Imaging and Cell Culture Laboratory (iCELL) is a full-service laboratory offered by the Central Instrumentation Facility under the Office of the Vice President for Research and Innovation. The facility houses advanced bioimaging and cell culture equipment, including the Confocal Laser Scanning Microscope (LEICA SP8-X FALCON with Live Cell Imaging), the Zeiss AxioImager Z2 Fluorescence Microscope with Cytogenetics Workstation, the BioTek Synergy HTX – Multimode Microplate Reader, as well as a fully functional Mammalian Cell Culture Laboratory.

The laboratory is located on the 3rd floor of Dr. George S.K. Ty Central Instrumentation Facility at DLSU Laguna Campus in Binan, Laguna.

What can we offer?

iCELL offers a variety of cell-based assays with the standard protocols, including those for:

  • Cytotoxicity Assays (MTT / Prestoblue)
  • Apoptosis Assay (Acridine Orange / Propidium Iodide Staining)
  • Fluorescence Microscopy

as well as several more assays in the pipeline coming soon, which include:

  • Scratch Wound Healing Assay
  • Chromosomal Analysis

iCELL also offers Live Cell Imaging to observe mammalian cells as they undergo specific treatments or modifications. This can be done under brightfield or with fluorescent dyes. 

 

As of May 2026, the cell lines available for study in iCELL’s portfolio include the following:

  • HT-29 (colorectal cancer)
  • MCF-7 (breast cancer)
  • A549 (lung cancer)
  • HEK293 (normal cell line, human embryonic kidney cells)
  • HDFn (normal cell line, human dermal fibroblasts)
  • Vero (normal cell line, monkey kidney epithelial cells)

 

For confocal fluorescence microscopy, our system uses two lasers. We have a 405 nm UV laser that can be used to view traditional blue dyes such as DAPI or Hoechst 33342. We also have a white light laser that can visualize dyes with excitations ranging from 470 – 670 nm.

How much do iCELL Services cost?

Analysis Type / Item DLSU Rates (Php)DLSP Rates (Php)Academic Rates (Php)Government Rates (Php)Industry Rates (Php)
MTT/PrestoBlue Assay Base rate (1 extract vs. 1 cell line)17,50020,00025,00028,75032,500
Additional cost for another extract (same cell line)8,75010,00012,50014,37516,250
Apoptosis Assay (AO/PI Dual Staining)Base rate (1 extract vs. 1 cell line)17,50020,00025,00028,75032,500
Additional 24-hour exposure for AO/EB(+) 3,500(+) 4000(+) 5,000(+) 5,750(+) 6,500
Package (MTT/PrestoBlue + AO/PI)Base rate (1 extract vs. 1 cell line)26,25030,00037,50043,12548,750
Instruments
CLSM per session (half-day)3,5004,0005,0005,7506,500
Inverted Brightfieldper session (half-day)1,0501,2001,5001,7251,950
Remarks

For Cytotoxicity and Genotoxicity Assays:

  • Samples must be submitted as soluble solid extracts with a minimum weight of one (1) gram.
  • Specimens intended for cytotoxicity or apoptosis evaluation must have completed a preparation sequence of crude extraction, rotary evaporation, and subsequent lyophilization or oven desiccation.
  • Standard rates apply to a single assay configuration involving one cell line and one extract; requirements for additional extracts, cell lines, or custom serial dilutions are subject to technical review and pricing adjustments.
  • Cytotoxicity protocols incorporate high-low positive controls (such as Triton-X) solely for procedural validation; these benchmarks are not intended for comparative activity analysis against the test extract. If you would like to run a parallel positive control substance against your extract, it will be charged as equivalent to a subsequent extract.
  • While the primary deliverable is the IC50 value for cytotoxicity, raw absorbance data as well as cell images throughout the assay are also provided to the client.
  • Apoptosis visualization is conducted via confocal fluorescence microscopy; iCELL provides the raw image files only, as analytical interpretation remains the responsibility of the client.
  • Any specimens involving hazardous biological agents or chemical treatments must be declared and reviewed with iCELL staff before arriving at the facility.
  • iCELL reserves the authority to decline processing if samples are found to possess hazardous traits during any stage of the request.
  • Due to IT security policies, external USB drives cannot be used for data transfer. Resulting files will be shared via email or through Google Drive for larger datasets.

For Brightfield / Fluorescence Microscopy:

  • Samples must be placed in standard microscope slides (75mm x 25mm) or in a 35mm petri dish. No other sample containers can be used as of the moment. Slide cover slips are necessary for fluorescence microscopy but remain optional for brightfield observations.
  • We do not have stocks of fluorescent dyes in our facility. Your samples must be already prepared and stained in your own laboratory before bringing them to us.
  • For specimens in petri dishes, culture flasks, or chamber slides, ensure the contact surface is free of debris or scratches to prevent imaging interference.
  • Standard analysis sessions are capped at three hours; exceeding this duration will result in extra charges based on the added workload and time.
  • Quoted rates cover a single capture setup. Projects requiring multiple captures over time, such as longitudinal experiments, are subject to pricing negotiations.
  • Any samples involving hazardous biological agents or chemical treatments must be declared and reviewed with iCELL staff before arriving at the facility.
  • iCELL reserves the authority to decline processing if samples are found to possess hazardous traits during any stage of the request.
  • Delivery and imaging schedules must be pre-arranged, and clients may attend the session remotely to guide the image capture process.
  • Due to IT security policies, external USB drives cannot be used for data transfer. Resulting images will be shared via email or through Google Drive for larger datasets.

 

Frequently Asked Questions

Do you allow external students/researchers to do the assay?

No. External students/researchers are not permitted to personally conduct experiments in our laboratory, even under supervision. Only our researchers and students under the residency program are authorized to perform the experiment. 

Do you offer rush analysis?

No. We do not offer rush analysis. All analyses are processed on a first-come, first-served basis.

How many samples should we submit for the cytotoxicity analysis?

We require a minimum of 1 gram of soluble solid form extracts after crude extraction, rotary evaporation, and freeze-drying or oven-drying. 

Do you have lab space for lab work prior to analysis?

No. We only accept prepared samples for analysis and do not provide laboratory space for sample preparation or other experimental work prior to submission.

Do you offer discounted rates for students?

Discounted rates are available only to students affiliated with DLSU and the DLSP network. For more information, please contact us at [email protected].

Do you offer training and workshops?

Yes. We offer training sessions and workshops related to our laboratory services. Please contact us for details at [email protected]

Do you provide certification after training and workshops?

Yes, certificates are provided after the completion of training sessions or workshops. At the moment, these trainings are not yet eligible for CPD units.

Your contact information is not working. Is there an alternative e-mail?

Yes. You may contact us through the following alternative e-mail addresses: 

Can we visit the iCELL facility at any time?

As part of university policy, all visitors must have a proper host on campus. We require prior coordination before visiting DLSU Laguna Campus. Please provide the purpose of the visit, the complete names of visitors, the preferred date and time, and vehicle details such as color, make, and plate number. 

Do you have a parking space at DLSU Laguna?

Yes, parking spaces are available at DLSU Laguna. Please coordinate with us in advance for the gate pass processing. We will require the visitor’s complete name, date and time of the visit, and vehicle details including color, make, and plate number.

How can we go to DLSU Laguna?

From Manila by private vehicle:

Take SLEX and exit via Sta. Rosa (or Eton), Proceed to Paseo de Sta Rosa. At the intersection of Sta. Rosa-Tagaytay Road and Laguna Blvd., continue along Laguna Blvd toward Laguna Technopark. After passing Laguna Technopark Gate 2, continue straight until you see a DLSU star signage (on the right). Turn left, pass underneath the CALAX road, and continue straight until the rotunda. The DLSU Laguna gate is located at the second exit.

From Manila by public transportation:

Take a bus bound for Sta. Rosa. Get off after the Sta. Rosa toll exit. Ride a tricycle going to Shopwise Paseo de Sta. Rosa, then take another tricycle for a special trip to DLSU Laguna.

Contact Us

DLSU Imaging and Cell Culture Laboratory (iCELL)

Central Instrumentation Facility

105 George Ty (GT) Building

De La Salle University Laguna Campus

Laguna Boulevard, LTI Spine Road, Barangays Biñan and Malamig, Binan, Laguna

e-mail: iCELL@dlsu.edu.ph