De La Salle University - Manila

Pathfinder :   Cloning

Scope Note :

"Cloning is the process of making a genetically identical organism through nonsexual means." (Source: http://www.howstuffworks.com/cloning.htm)

"The word cloning has several different meanings. For example, it is possible to clone cells, that is, to cause cells to reproduce themselves so as to make a population of identical cells. In molecular biology, any piece of DNA can be cloned-inserted into a vector that replicates in a host to produce many copies of the same recombinant vector. It is also possible to clone genes. Gene cloning is the process of identifying and isolating a specific gene of interest. Cloning genes is a major focus of molecular biology, and the ability to clone genes has revolutionized biology. Once a gene is identified and cloned into an appropriate vector, it can be manipulated in many different ways. Cloning dramatically amplifies the DNA so that it can be sequenced. A cloned gene inserted into an expression system creates an organism that produces up to 25% of its total protein or more as the gene product, allowing large-scale production of the protein." (Source : Encyclopedia of Molecular Biology. New York : Wiley, 1999. QH 506 C74 1999)

To access the collection, use the following subject headings:

• Clone cells
• Cloning
• Cloning -- Government policy -- United States
• Cloning -- Moral and ethical aspects
• Cloning -- Social aspects
• Human cloning
• Molecular cloning

The following related terms maybe helpful in locating additional materials:

• Bioethics
• Biotechnology
• Developmental biology
• DNA
• Genetic engineering
• Genetics
• Medical ethics
• Molecular biology
• Reproduction, Asexual
• Stem cells

BOOKS

Basic references located at the Information-Reference Section, 2nd floor :

• DNA cloning : a practical approach. QH 442.2 D19 1995
• Cloning, gene expression, and protein purification : experimental procedures and process rationale. Charles C. Hardin QH 442.2 C545 2001
• Encyclopedia of molecular biology. QH 506 C74 1999
• The human genome. QH 447 H85 2001
• Human genome methods. QH 431 H85 1998
• Methods in gene biotechnology. QH 442 M47 1997
• Recombinant DNA and biotechnology : a guide for students. Helen Kreuzer and Adrianne Massey. QH 442 K7 2001

Texts located at the Circulation Section, 3rd floor:

• The baby makers. Diana Frank and Marta Vogel. QP 251 F74 1988
• Biophysical modelling of radiation effects. QH 652 B56 1992
• The clonal basis of development. QH 511 S6 1978
• Cloning : a biologist reports. Robert Gilmore McKinnell QH 442.2 M34 1979
• Cloning : for and against . QH 442.2 C54 1999
• The cloning of man : a brave new hope--or horror? QH 442.2 C55 1978
• Crafting a cloning policy : from dolly to stem cells. Andrea L. Bonnicksen QH 442.2 B66 2002
• DNA replication. Roger L.P. Adams. QP 624 A3 1991
• Essential molecular biology : a practical approach. QH 442.2 E77 2000
• Ethics and law in biological research. QH 438.7 E8 2002
• Genetic dilemmas : reproductive technology, parental choices, and children's futures. Dena S. Davis. RG 133.5 D38 2001
• The human body shop : the cloning, engineering, and marketing of life. Andrew Kimbrell. TP 248.2 K55 1997
• Human cloning : playing God or scientific progress? Lane P. Lester and James C. Hefley. QH 442.3 L47 1998
• Methods in molecular biology and protein chemistry : cloning and charecterization of an enterotoxin subunit. Brenda D. Spangler. QP 519.7 S6 2002
• Private choices, public consequences : reproductive technology and the new ethics of conception, pregnancy, and family. Lynda Beck Fenwick. RG 133.5 F46 1998
• Reproducing persons : issues in feminist bioethics. Laura M. Purdy. RG 133.5 P87 1996
• Reproductive technologies, marriage and the church. RG 133.5 R46 1988
• Understanding DNA and genecloning : a guide for the curious. Karl Drlica. QH 442.2 D75 1992

AUDIO VISUAL MATERIALS
(Instructional Media Services, Ground floor)

• CNN Perspective : cloning. [V001827]
• DNA & RNA : deciphering the code of life. [V002368]
• The ethics of cloning. [V001732]
• Geometry of life : DNA.   [QH430]
• The living cell : DNA.   [CD02834]
• Mitosis and meiosis : how cells divide. [V002375]

PERIODICALS

ELECTRONIC

Proquest Online
http://www.umi.com/pqdauto

• American Biology Teacher
• American Scientist
• Bioscience
• Genomics & Genetics Weekly
• Health & Medicine Week
• The Journal of Nutrition
• The Lancet
• Medical Devices & Surgical Technology Week
• Perspectives in Biology and Medicine
• Science
• Systematic and Applied Microbiology

Science Direct
http://www.sciencedirect.com

• FEBS Letter
• Journal of Bioscience and Bioengineering
• Journal of Molecular Catalysis B : Enzymatic

VERTICAL FILE
(Information-Reference Section)

• The ethics of human cloning. J.Q. Wilson. VF 01855
• Reflections on cloning. VF 01624
• Towards development of a national policy on human cloning. M.V.T. Reyes. VF 01274

Faculty Publications
(Archives Section, 4th floor)

• Technolites : DNA fingerprinting and DNA computer. Estalila, Aliento V. Manila Billetin. May 14, 2002
• Worldview: heightened debates on human cloning. Villacorta, Wilfrido V. Manila Bulletin. December 5, 2001.

THESES AND DISSERTATIONS

LOCAL
(Archives Section, 4th floor)

• The advent of cloning: a redefinition of certain concepts. Mary Rose S. Santos (1994) TU 06423
• To clone or not to clone : that is the ethical question. Aimee Margaret Po (2003) TU 11348

FOREIGN
Proquest Digital Dissertations
http://www.umi.com/pqdauto 

INTERNET WEBSITES
(Cybernook, Ground floor; Graduate corners, 2nd and 3rd floors)

Human Cloning. Org
http://www.humancloning.org/
HumanCloning.org is the official site in support of human cloning technology. It contains news and introductory essays on the benefits of human cloning, all the reasons to clone human being, the top 10 myths about human cloning and human cloning is the cure for infertility.

Human Genome Project Information
http://www.ornl.gov/sci/techresources/Human_Genome/elsi/cloning.shtml
This page contains quick links to questions and answers about human genome project such as information on cloning, different types of cloning, cloning technologies, the risk of cloning, etc. Related links on general cloning information, cloning in the news, cloning ethics, policy and legislation, cloning problems, cloned animals, cloning for organs and more are included.

United States Conference of Catholic Bishops. Prolife Activities
http://www.nccbuscc.org/prolife/issues/bioethic/clonfact202.htm
This page includes discussion on what human cloning is. In the public policy debate on human cloning, groups that support the cloning of human embryos for destructive research have raised questions as to what procedures are properly called human cloning.

Should you have commnets or suggestions on this pathfinder, please call us at these telephone numbers, 536-0244, 524-4611 local 620 or emails us through "Ask LORA".

Date: February 2004 / March 2010